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Comprehensive Digestive Stool Test – GI MAP Pathogen Plus.

Comprehensive Digestive Stool Test – GI MAP Pathogen Plus.

Organisms in the digestive tract, such as bacteria, parasites and viruses can be the underlying cause of many digestive conditions and illnesses.

About this test

The GI-MAP is the first and only gut test in the world to be running full quantitative qPCR, meaning DNA testing on multiple Gastro intestinal targets, including pathogens, bacteria, worms, yeasts, parasites and comprehensive antibiotic resistant genes, alongside intestinal health and immune markers.

The GI-MAP was designed to assess a patient’s microbiome ( gut bacteria )  from a single stool sample, with particular attention to microbes that cause disease or that disrupt normal microbial balance and contribute to changes in the GI flora and contribute to illness. With full quantification, we can now see the level of infection thus, the GI-MAP arms us with accurate, actionable information.

What is being measured

A summary of what is being tested:

  • Pathogenic bacteria, toxins, parasites and viruses
  • Commensal / Normal bacteria including Bacteroides fragilis group, Bifodobacter, Enterococcus, bifido & Lactobacillus
  • Dysbiotic Bacteria – including Morganella morganii, Pseudomonas species & Streptococcos species
  • Bacterial Autoimmune Triggers – including Proteus mirabilus, Proteus spp & Klebsiella pneumoniae
  • Opportunistic Parasites – including Blastocystic Hominis, Dientamoeba Fragilis & Endolimax nana
  • Worms
  • Yeasts, fungi and moulds;  including the Candida species, Cyclospora, Geotricum species & Trichosporon species
  • Helicobacter pylori and virulence factors
  • Phyla microbiota ratios
  • GI Health Markers including;
    Secretory IgA (sIgA)
    Anti-gliadin sIgA
    Pancreatic elastase 1
    Steaotocrit
    Beta-glucuronidase
    Calprotectin
    Faecal Occult blood

For the full list of what is being measured, see the sample report at the bottom of the page.

A little about a few of the markers in the test.

Blastocystis hominis – also tested using PCR  – which checks for the DNA of the parasite.

Blastocystia hominis is a parasite commonly found in the GI tract. Symptoms of infection include:

  • Diarrhoea
  • Nausea
  • Abdominal cramps
  • Bloating
  • Gas
  • Weight loss
  • Fatigue

Candida species are constituents of a normal healthy gut flora however if an overgrowth of the yeast (candidosis) occurs, gut permeability can increase leading to leaky gut syndrome. This can allow the yeast hyphae to penetrate the gut wall lining resulting in potential systemic infections.

Helicobacter pylori is commonly thought to be the bacteria responsible for causing stomach ulcers, gastritis and virtually all abdominal ulcers, apart from drug-induced forms. It survives the acidic environment of the stomach and infection can lead to pain and inflammation, which over time can significantly increase the risk of gastric cancer. There is also the h. pylori breath test available by following the link.

Optional Add-on

You can opt to add on zonulin family peptide to this test if you prefer. Zonulin is gut a marker associated with intestinal permeability / Leaky gut. Research by Fassano concluded that zonulin acted like a shoe lace to pull the cells of the intestines together. If it was found outside of the reference range it was concluded that it was associated with a leaky gut or intestinal permeability. A recent research paper published in Frontiers in Endocrinology suggested that the zonulin kits from Immundiagnostik (IDK) ( who supply all the labs that we know that run zonulin) did not detect zonulin (a precursor of Haptoglobin 2). This issue was further confirmed by the kit manufacturer, Immundiagnostik. Because some researchers are conducting studies and have received data from the current zonulin kits, I am still offering zonulin but have renamed it Zonulin family peptide.

The Scheffler paper suggests that the kits in question may detect properdin, a protein involved in the alternative complement pathway and inflammation. Preliminary study results from an external investigator suggest that properdin may be structurally and functionally similar to zonulin. When analysing data it has been found there is a high correlation with raised zonulin and other markers associated with gut mucosal health.

Age range this test is suitable for

Suitable for all ages. Children need to be potty trained and out of nappies. The sample must not be contaminated with urine or fibres from nappies.

The GI-MAP can be run on a patient of any age, from infants to adulthood. The main thing to bear in mind when testing for a child under the age of 2 is that the results will more indicative of any issues, rather than an accurate reading of the overall health of the microbiome. This is because in children under 2, the microbiome has not fully developed yet and so this would need to be considered during the analysis of the report

Specimen required

1 single stool sample

Before taking the test

You will need a break after you have finished any anti-microbial programmes before being able to complete this test otherwise you risk having false negative results. The length of the break depends on the medication. We often recommend 2 weeks after antibiotics or antimicrobials.

Sample Return
Samples can be sent Monday – Wednesday only to arrive at the lab the following day. Please check there isn’t a bank holiday if the Monday falls on day 3 of your collection.

European customers will receive their test kits via courier delivery. A return courier collection of the samples is included in the shipping fee of £30. Samples can be sent Monday – Wednesday only to arrive at the lab the following day.

Non European customers please email sue@smartnutrition.co.uk to make an enquiry.

Turnaround time
21  days

All sample reports are for representational and educational purposes only. Biomarkers, references ranges, results, and all other data may differ from actual reports. All data included in no way represents an actual patient. Any comparisons of results to actual patients, is completely incidental. 

Smart Nutrition GI-Map Pathogen Plus Sample Report

GI Map White Paper Dec 2017