DUTCH Plus Test – Comprehensive Hormone Testing with Cortisol Awakening Response (CAR) for Women

The DUTCH Plus Test is an advanced hormone panel that provides a complete picture of your adrenal and sex hormone health. Designed specifically for women, this comprehensive test analyses four key areas: sex hormones and their metabolites, adrenal function, organic acid markers, and the Cortisol Awakening Response (CAR).

Unlike the DUTCH Complete, the DUTCH Plus goes one step further by mapping your body’s response to stress through detailed CAR tracking, offering deeper insights into your HPA axis function. This non-invasive, at-home test helps uncover the root causes of hormonal imbalances, energy issues, and stress-related symptoms, empowering you to take control of your hormonal health with clarity and precision.

What is the DUTCH Plus Test?

The DUTCH Plus Test is a comprehensive hormone and adrenal assessment that measures four key areas of health: sex hormones, adrenal function, organic acid markers, and the Cortisol Awakening Response (CAR). It offers a deeper, more complete analysis than the DUTCH Complete by including CAR to map stress response in greater detail.

DUTCH Sex Hormones

The DUTCH Sex Hormone panel looks at hormone levels and their breakdown, including oestrogen metabolites, androgen metabolites (testosterone, DHEA, DHT), and progesterone metabolites. As well as raw data, results are presented in a clear graphical format, making it easier to understand hormonal pathways and any imbalances.

DUTCH Adrenal

This panel assesses daily free cortisol and cortisone levels, alongside metabolites of cortisol and DHEA. It includes creatinine correction for the four sample points collected throughout the day, providing a detailed picture of adrenal rhythm and stress hormone production.

DUTCH Organic Acid Tests (OAT)

The Organic Acid panel offers insight into key nutritional and metabolic markers. It includes:

  • Vitamin B6, B12, and biotin markers
  • Glutathione (for detoxification)
  • Gut dysbiosis marker (indican)
  • Neurotransmitter metabolites (dopamine, norepinephrine)
  • Melatonin and oxidative stress markers

These markers reflect overall biochemical balance, energy production, and neurotransmitter activity.

DUTCH Cortisol Awakening Response (CAR)

Unique to the DUTCH Plus, the CAR measures cortisol levels at waking and over the following hour. This provides a dynamic picture of adrenal function and HPA axis activity, helping to pinpoint specific stress-response imbalances. It also maps daily cortisol and cortisone patterns for a more complete view of adrenal health.

Should You Take the DUTCH Plus Test?

The DUTCH Plus Test is ideal if you’re experiencing symptoms of hormonal imbalance, such as:

It is also highly valuable if you’re on Hormone Replacement Therapy (HRT), as it helps evaluate how your body is metabolising hormones, particularly oestrogen, highlighting any potential for build-up or imbalance.

The key advantage of the DUTCH Plus over the DUTCH Complete is the inclusion of the Cortisol Awakening Response (CAR). Measured at five points throughout the day, CAR offers a detailed view of adrenal health by tracking how your cortisol levels rise and fall after waking.

This pattern acts as a “mini stress test,” revealing imbalances in your hypothalamic-pituitary-adrenal (HPA) axis that may be contributing to fatigue, anxiety, or poor stress resilience.

How the Organic Acids Test Works

  1. Your test kit will be delivered directly to your home.
  2. Collect a urine sample following the instructions provided. Full, easy-to-follow instructions are included with your kit.
  3. Return your sample using the prepaid courier label included in the kit.
  4. Receive your results by email within 12 working days.

Special Instructions

  • This test is suitable for women who have reached puberty and either have a menstrual cycle or wish to monitor hormone replacement therapy (HRT).
  • Follow the collection instructions provided in your test kit. These vary based on whether you have regular or irregular periods.
  • Two weeks before testing: Stop tyrosine supplements (if advised by your practitioner).
  • Two days before testing: Discontinue vitamin B6 and other vitamins/minerals.
  • 48 hours before testing: Avoid avocados, bananas, and fava beans.
  • Inform your practitioner if you are taking tyrosine, L-Dopa, DLPA, mucuna, or quercetin, as these may affect results.

Order Your DUTCH Plus (with CAR) Test Today

Gain deeper insight into your hormonal health and stress response with the DUTCH Plus Test. With detailed analysis of sex hormones, adrenal function, and the cortisol awakening response, this test provides valuable data to guide personalised, effective care.

Take control of your well-being. Order your DUTCH Plus Test now.

FAQs

The DUTCH Plus (adrenal and sex hormone) for Women test measures the key female hormones oestrogen and progesterone, along with the stress hormones cortisol and DHEA-S. It also measures testosterone plus the breakdown products of the above. It covers 35 different hormones.

  • Oestrogen, progesterone and testosterone – the important sex hormones
  • Daily free cortisol including 4 different samples taken over 24 hours providing information about stress patterns throughout the day
  • Oxidative stress marker (8OHdG) providing information about free radicals and damage to cells
  • Melatonin  – the major modulator of circadian rhythms in the body associated with the sleep / wake cycle
  • Organic acids including markers for vitamin B12 (methylmalonate), vitamin B6 (xanthurenate), glutathione (pyroglutamate), dopamine (homovanillate), adrenaline (vanilmandelate), and serotonin (5-hydroxy indole acetate). They provide information about metabolism/workings in the body’s pathways.
  • Cortisol Awakening Response – an indicator of the adrenal function with the natural rise of cortisol in the morning measured.

This test is suitable for any women wanting to investigate possible hormonal imbalances or hormone related conditions such as:

  • Menopause
  • PCOS – polycystic ovary syndrome
  • Osteoporosis
  • Amenorrhea  – no periods
  • Weight gain
  • Fatigue
  • Low libido
  • Premenstrual syndrome
  • Mood swings
  • Sleep issues
  • Mood disorders
  • Endometriosis
  • Fibroids
  • Anovulatory cycle – not ovulating
  • Oestrogen dominance
  • Monitoring hormone replacement therapy

In addition to the hormone conditions mentioned above, this test is really useful to anybody who is potentially suffering from a low or elevated Cortisol Awakening Response. Symptoms could include but not limited to:

  • Excessive psychological burnout
  • Seasonal affective disorder (SAD)
  • Sleep apnoea
  • Poor sleep
  • Post Traumatic Stress Disorder
  • Chronic Fatigue
  • Chronic Pain
  • Chronic stress
  • Glycaemic dysregulation
  • Depression

Other areas that this could be implicated, include:

  • Systemic hypertension
  • GI diseases
  • Postpartum depression
  • Autoimmune diseases
  • 16-OH-E1
  • 2-Methoxy-E1
  • 2-OH-E1
  • 2-OH-E2
  • 24 hour free cortisol
  • 24 hour free cortisone
  • 4-OH-E1
  • 4-OH-E2
  • 5-Hydroxyindolacetate (5HIAA)
  • 5a-Androstanediol
  • 5a-DHT
  • 5b-Androstanediol
  • 8-hydroxy-2′-deoxyguanosine (8-OHdG)
  • a-Pregnanediol
  • a-Tetrahydrocortisol (a-THF)
  • Androsterone
  • b-Hydroxyisovalerate
  • b-Pregnanediol
  • b-Tetrahydrocortisone (b-THE)
  • b-Tetrahyrodcortisol (b-THF)
  • Cortisol (saliva) x 5
  • Cortisone (saliva) x 5
  • DHEA-S
  • DHT
  • Epi-Testosterone
  • Estradiol (E2)
  • Estriol (E3)
  • Estrone (E1)
  • Etiocholanolone
  • Homovanillate (HVA)
  • Indican
  • Melatonin
  • Metabolized cortisol (THF+THE)
  • Methylation activity (2-Me-E1 / 2-OH-E1)
  • Methylmalonic Acid (MMA)
  • Progesterone
  • Progesterone (serum equivalent)
  • Pyroglutamate
  • Quinolinate (neuroinflammation marker)
  • Testosterone
  • Total DHEA
  • Total Oestrogen (Estrogen)
  • Vanilmandelate (VMA)
  • Xanthurenate

 

 

 

DUTCH stands for Dried Urine Test for Comprehensive Hormones – it’s a urine hormone test.

The DUTCH Plus test is simple and easy to do. Urine samples are taken using filter paper, making the test suitable for use at home. The DUTCH Plus also incorporates five salvia samples throughout the day.

15 years and above.

14 working days.

 

 

 

Your test results will be emailed to you.

Simply collect the sample as instructed the following day. All samples do not need to be collected in one 24-hour period.

No, they can be collected in a different order. If you wish, you may start with sample #3, followed by #4, #1 & #2. If you begin with #3, collect the extra sample if you wake and urinate in the night.

While hormone levels are very stable in dried samples, they should be sent back as soon as possible. If you have to wait to send them in, place them in the freezer (in a plastic bag) after drying.

This test is built to test patients “on” their hormones. Our suggestion is to follow the Hormone Schedule (listed previously) and any specific instructions given by your provider.

Collect the bedtime sample (#2) before your longest stretch of sleep, the waking sample (#3) after this sleeping period, and sample #4 two hours later. The dinnertime sample (#1) should be collected 4-7 hours before bed.

It’s advisable to wait three cycles before testing after you’ve come off the pill.

This sample report includes the bile acids and SCFA’s add on

Available Add-ons

ORDER YOUR TEST

related tests

This profile provides insight into the body’s ability to manage stress by assessing the hormones cortisol and DHEA. Imbalances in these hormones can result in a wide range of symptoms from fatigue to loss of motivation, depression and hormonal problems.

 

The NutrEval test is the most comprehensive health MOT available. It identifies nutritional deficiencies and insufficiencies for over 125 markers and provides personalised recommendations for each.

The Oestrogen Metabolism Assessment Test evaluates how oestrogen is being processed in the body. The Oestrogen Metabolism Test is designed for both premenopausal and postmenopausal women.

A one day progesterone and oestradiol test that provides levels of these hormones. This is suitable for menopausal women and maybe used to track hormone treatments and manage menopausal symptoms.

PUT YOURSELF to the TEST

Please do not return samples to the laboratories that may arrive after Wednesday 27th March and up to and including Monday 2nd April.

The laboratories are closed from the 28th March – 2nd April for the Easter Holiday.