DUTCH Test – Complete Dried Adrenal and Sex Hormone Test For Women

DUTCH Test – Complete Dried Adrenal and Sex Hormone Test For Women

The DUTCH Test is a comprehensive assessment of sex and adrenal hormones and their metabolites.

What does the DUTCH Test measure?

The DUTCH Complete test (adrenal and sex hormone) can help identify the root causes of hormone imbalances in both men and women. The test includes markers providing information about adrenal (stress) hormones as well as reproductive hormones (oestrogen, progesterone and testosterone).

Balanced levels of sex hormones throughout life are crucial for optimal health for both men and women. Sex hormones influence reproductive capability and sexual characteristics. They contribute to the determination of our body shape, sexual character, motivation, behaviour and fertility. They fluctuate throughout life and imbalances in these pathways can result in a variety of symptoms.

The sex hormones oestrogen, progesterone and testosterone belong to the steroid hormone group, which also includes DHEA, testosterone and cortisol. They work together to influence cellular physiology in many ways through connected pathways.

The DUTCH test can also be used to gather baseline measurements for the administration and monitoring of hormone replacement therapy.

Should you do a DUTCH Test?

If you’re experiencing a hormonal imbalance, you may notice symptoms such as irregular menstruation (including heavy or painful periods), PMS, headaches, reduced libido, bloating, mood swings, fatigue, anxiety, depression, breast tenderness, and conditions like endometriosis, fibroids, acne, or weight gain all of which can be influenced by hormones, Completing the DUTCH Test will give you valuable information

When undergoing Hormone Replacement Therapy (HRT), it’s vital to consider how your body metabolizes these hormones and assess the potential risk of estrogen buildup.

Who is the DUTCH Test Suitable for?

The DUTCH Test is suitable for all women that want to have a look at their hormone levels and the impact on their menstrual cycle and well-being. The Dutch test can be used when you are taking an oral contraceptive pill or if you are using a Mirena Coil.

The DUTCH test can be used when you are menopausal and it is suitable for monitoring Hormone Replacement Therapy (HRT)

At Smart Nutrition we pride ourselves in offering the best cost possible for the DUTCH Test – complete dried adrenal and sex hormone test for women.

The DUTCH Complete (adrenal and sex hormone) for Women test measures the key female hormones oestrogen and progesterone, along with the stress hormones cortisol and DHEA-S. It also measures testosterone plus the breakdown products of the above. It covers 35 different hormones.

  • Oestrogen, progesterone and testosterone – the important sex hormones
  • Daily free cortisol including 4 different samples taken over 24 hours providing information about stress patterns throughout the day
  • Oxidative stress marker (8OHdG) providing information about free radicals and damage to cells
  • Melatonin  – the major modulator of circadian rhythms in the body associated with the sleep / wake cycle
  • Organic acids including markers for vitamin B12 (methylmalonate), vitamin B6 (xanthurenate), glutathione (pyroglutamate), dopamine (homovanillate), adrenaline (vanilmandelate), and serotonin (5-hydroxy indole acetate). They provide information about metabolism/workings in the body’s pathways.

This test is suitable for any women wanting to investigate possible hormonal imbalances or hormone related conditions such as:

  • Menopause
  • PCOS – polycystic ovary syndrome
  • Osteoporosis
  • Amenorrhea  – no periods
  • Weight gain
  • Fatigue
  • Low libido
  • Premenstrual syndrome
  • Mood swings
  • Sleep issues
  • Mood disorders
  • Endometriosis
  • Fibroids
  • Anovulatory cycle – not ovulating
  • Oestrogen dominance
  • Monitoring hormone replacement therapy
  • 16-OH-E1
  • 2-Methoxy-E1
  • 2-OH-E1
  • 2-OH-E2
  • 24 hour free cortisol
  • 24 hour free cortisone
  • 4-OH-E1
  • 4-OH-E2
  • 5-Hydroxyindolacetate (5HIAA)
  • 5a-Androstaendiol
  • 5a-DHT
  • 5b-Androstanediol
  • 8-Hydroxy-2′-deoxyguanosine (8-OHdG)
  • a-Pregnanediol
  • a-Tetrahydrocortisol (a-THF)
  • Androsterone
  • b-Hydroxyisovalerate
  • b-Pregnanediol
  • b-Tetrahydrocortisone (b-THE)
  • b-Tetrahyrodcortisol (b-THF)
  • Cortisol (free) x 4
  • Cortisone (free) x 4
  • DHEA-S
  • Epi-Testosterone
  • Estradiol (E2)
  • Estriol (E3)
  • Estrone (E1)
  • Ethylmalonic
  • Etiocholanolone
  • Homovanillate (HVA)
  • Indican
  • Kynurenate
  • Melatonin
  • Metabolized cortisol (THF+THE)
  • Methylation activity (2-Me-E1 / 2-OH-E1)
  • Methylmalonic Acid (MMA)
  • Progesterone
  • Progesterone (serum equivalent)
  • Pyroglutamate
  • Quinolinate (neuroinflammation marker)
  • Testosterone
  • Total DHEA
  • Total oestrogen (estrogen)
  • Vanilmandelate (VMA)
  • Xanthurenate


DUTCH stands for Dried Urine Test for Comprehensive Hormones – it’s a urine hormone test.

The DUTCH test is simple and easy to do. Urine samples are taken using filter paper, making the test suitable for use at home.

Before taking the DUTCH test female patients must have gone through puberty and have a menstrual cycle.

Please read the instructions that are included with the DUTCH test. The instructions explain the collection schedule for women with regular and irregular periods, no periods, and peri-menopausal and menopausal women.

48 hours prior to sample collection you will need to avoid the following foods: avocados, bananas and fava beans.

Some supplements may impact the result. If you take any of the following, please let your practitioner know: tyrosine, L-Dopa, D,L-Phenylalanine (DLPA), mucuna and quercetin.


12 years and above.

Please return using the pre paid label and shipping instructions included with your test kit.


12 working days.

Your test results will be emailed to you.

What if I miss a collection?
Simply collect the sample as instructed the following day. All samples do not need to be collected in one 24-hour period.

Do I have to take the samples in the order listed?
No, they can be collected in a different order. If you wish you may start with sample #3, followed by #4, #1 & #2. If you begin with #3, collect the extra sample if you wake and urinate in the night.

How long can I keep the dried samples before sending them in?
While hormone levels are very stable in dried samples, they should be sent back as soon as possible. If you have to wait to send them in, place in freezer (in plastic bag) after drying.

Do I need to stop taking my hormones for this test?
This test is built to test patients “on” their hormones. Our suggestion is to follow the Hormone Schedule (listed previously), and any specific instructions given by your provider.

What if my sleep schedule is abnormal (night workers, etc.)?
Collect the bedtime sample (#2) before your longest stretch of sleep, the waking sample (#3) after this sleeping period, and sample #4 two hours later. The dinnertime sample (#1) should be collected 4-7 hours before bed.

What if I am unable to urinate at the specific time?
Simply drink some fluids and go as soon as you are able.

DUTCH Test – Complete Dried Adrenal and Sex Hormone Test For Women

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Please do not return samples to the laboratories that may arrive after Wednesday 27th March and up to and including Monday 2nd April.

The laboratories are closed from the 28th March – 2nd April for the Easter Holiday.