Essential Oestrogens

Essential Oestrogens

The Oestrogen Metabolism Assessment evaluates how oestrogen is being processed in the body.

A balance of anti- and pro-carcinogenic breakdown products of oestrogen are assessed. This sheds light into many oestrogen-dependent conditions and provides an important tool for monitoring dietary, lifestyle and hormone therapies.

If you have a family history of hormonal cancers such as breast or uterine cancer, or a history of oestrogen dominance which can result in conditions such as PMS, fibroids and endometriosis, then this test could be of value to you.

Oestrogen metabolism

Oestrogen metabolites provide valuable clinical information regarding cancer risk. Two significant metabolites (breakdown products) that can be accurately assessed are 16 alpha-hydroxyestrone (16 alpha-OHE1) and 2-hydroxyestrone (2-OHE1).

16 alpha-OHE1 is a powerful metabolite that stimulates target tissues. Levels can rise in response to obesity, alcohol consumption and toxic exposure. High levels of this potent metabolite are linked to increased risk and poorer prognosis in conditions linked to oestrogen excess such as breast cancer and lupus.

2-OHE1 is a metabolite that binds weakly to cell receptors and may slow cell proliferation. Excessive levels of 2-OHE1 may increase the risk of osteoporosis in postmenopausal women with low oestrogen.

The ratio between 2-OHE1 and 16 alpha-OHE1 is the key to optimising health.

Treatments that increase the 2:16 alpha-OHE1 ratio help to reduce the risk of oestrogen-dependent disease enabling correct dietary and supplemental choices and monitoring. The Oestrogen Metabolism Assessment is designed for both premenopausal and postmenopausal women.

The potential impact of oestrogen on a person’s health and wellbeing is enormous.

Oestrogen can exert a strong influence in diverse conditions, including:

  • Breast cancer
  • Prostate cancer
  • Osteoporosis
  • Heart disease
  • Autoimmune disorders


Estrone (E1)


Estriol (E3)

Estrogen Metabolites

2-Hydroxyestrone + 2-Hydroxyestradiol [2-OH(E1+E2)]

16α-Hydroxyestrone (16α-OH E1)

4-Hydroxyestrone+4-Hydroxyestradiol [4-OH(E1+E2)]

2-Methoxyestrone+2-Methoxyestradiol [2MeO(E1+E2)]

4-Methoxyestrone+4-Methoxyestradiol [4MeO(E1+E2)]


2-OH(E1+E2) / 16α-OHE1

2-OH(E1+E2) / 2-MeO(E1+E2)

Urine sample.


Premenopausal women evaluating ovulatory function: the urine needs to be collected between days 19 and 25 of the menstrual cycle.

Menopausal women can collect the specimen on any day.

Women who are taking hormone replacement therapies or take oral contraceptives can continue to take these as normal.

48 hours prior to collecting your sample, avoid eating whole or ground flax seeds and sesame seeds.

24 hours prior to collecting your sample, avoid dilution of urine sample by consuming no more than 1/2 oz water/fluid per pound of body weight per day. For example: 130 lbs -65 oz (eight 8 oz glasses).

The patient must have gone through puberty and have a menstrual cycle.

Please return via a next day service Monday – Thursday only. The sample must be stored in the fridge prior to returning and returned within 48 hours of collection.

A courier option is sent with your test kit. You pay the laboratory directly for this test and also the return courier if you use the service. You can also make your own arrangements for returning your samples via a next day service.

23 working days.

Your test results will be emailed to you.


Essential Oestrogens